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Abstract of final report on the
Australian Flora Foundation funded project:
In vitro propagation of Australian Proteaceae (Conospermum)
Eric Bunn, Kings Park and Botanic Garden, Kings Park West Perth 6005
July 8 1994
Grant details Final
report
Abstract
In vitro methods are investigated for four species of the Australian genus
Conospermum (smokebush): C. fioribundum, C. incurvum,
C. stoechadis and C. triplinervum. Shoots are multiplied
on a 1/2 MS medium supplemented with 5.µM kinetin and 0.5 µM
BA. Multiplication rates vary between species but addition of cytokinins
effectively doubles or trebles the multiplication rate. Shoots are elongated
by alternating incubation periods on media without plant hormones or containing
up to 3 µM GA. Elongated shoots are then rooted on medium containing
auxins (10 µM NAA or 5 µM NAA + 5 µM IBA for best results
with Conospermum stoechadis and C. triplinervum). Rooted
shoots are acclimatized before transfer to pasteurized potting medium
(coarse sand, peat; perlite in equal proportions), resulting in a creditable
survival rate for Conospermun triplinervum, C. stoechadis
and C. incurvum.
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